In situ extraction (milking) of the two promising Botryococcus braunii strains Showa and Bot22 under optimized extraction time

Abstract In situ extraction or “milking” of microalgae is a promising approach to reduce downstream costs in order produce low-value substances such as lipids from an economical way. Due its ability secrete high amounts long-chain hydrocarbons extracellular matrix, the green microalga Botryococcus braunii suitable for process cost intensive steps harvesting, dewatering, and cell disruption could be omitted. Based on previous study investigating various B. strains terms growth, lipid accumulation, solvent compatibility, Showa Bot22 (both B race) were identified potential candidates extraction. prove suitability these two extraction, this first determined optimal time using short-term over 7 days at different starting biomass concentrations 1.5 2.5 g L ?1 . Furthermore, both treated applying long-term extractions 30 confirm results extractions. The indicate strain-dependent 300 min day strain 200 Bot22. During days, hydrocarbon productivity was 16.99 mg (10.53 DW ) 14.53 (10.48 direct correlation between achieved by concentration respective established. It shown that consideration effective phase boundary area required calculate system independent value comparison setups.